Description
Intended Uses: Human PAI-1 tPA complex assay is intended for the quantitative determination of the covalent complex of tPA and its inhibitor PAI-1 in human plasma and other biological fluids.
Principle of the Assay: Human tPA in samples will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-human PAI-1 primary antibody binds to PAI-1 tPA complex captured on the plate. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standar calibration curve is prepared along with the samples to be measured using dilutions of CPAI tPA complex. Color development is proportional to the concentration of PAI-1 tPA complex in the samples. Free tPA and PAI-1 will not be detected by this assay.
Background: Tissue-type plasminogen activator (tPA) is a serine protease that activates plasminogen to plasmin in the blood fibrinolytic system. Plasminogen activator inhibitor type 1 (PAI-1) is involved in the regulation of the blood fibrinolytic system and forms a 1:1 covalent complex with tPA and tPA